How to Test Nitric Oxide Levels

At home and medical methods for testing NO levels

Nitric oxide (NO) is an element produced in your body that plays an important part in your cardiovascular health, and it may also measure the level of inflammation in your body. Low levels of NO may lead to high blood pressure, circulation problems, and decreased energy, but the most common reason to test is to evaluate asthma that doesn’t respond to medications. If you experience these symptoms, you can monitor your NO levels at home by using test strips that measure the NO in your saliva. If your NO levels are low, see your doctor for advice on how to raise them. In some cases, your doctor may conduct an exhaled nitric oxide test to diagnose asthma.

Method 1

Method 1 of 3:

Using PH Test Strips

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1
Buy nitric oxide test strips. There are several brands of commercially-available test strips that measure the levels of nitric oxide found in the saliva. These levels are supposed to indicate how much circulatory nitric oxide is being produced in your body. Purchase these strips online, or at select pharmacies and health stores.^{[1]XTrustworthy SourcePubMed CentralJournal archive from the U.S. National Institutes of HealthGo to source}
- Keep in mind that test strips might not be the best indicator of what is actually occurring in your body.
2
Place the test strip on your tongue for 5 seconds. Nitric oxide PH test strips have a saliva absorption pad on 1 end and a test pad on the other. Place the absorption pad flat on your tongue. Remove it after 5 seconds.^{[2]XTrustworthy SourcePubMed CentralJournal archive from the U.S. National Institutes of HealthGo to source}
- Don't let the test pad side of the strip touch your tongue.
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After removing the strip from your tongue, fold it in half. Make sure that the part that touched your tongue presses onto the test pad. Hold the end together for 5 seconds.^{[3]XTrustworthy SourceScience DirectOnline archive of peer-reviewed research on scientific, technical and medical topicsGo to source}
4
Refer to the color chart for your results. The packaging of the test strip should feature a color chart illustrating the possible range of nitric oxide levels measured by the strips. If the test pad turns a dark color, that means that you have high levels of nitric oxide in your body. If the color is light, your nitric oxide levels are somewhat depleted.^{[4]XTrustworthy SourcePubMed CentralJournal archive from the U.S. National Institutes of HealthGo to source}
- Nitric oxide levels under about 25 parts per billion in adults are considered normal.

Method 2

Method 2 of 3:

Taking an Exhaled Nitric Oxide Test for Asthma

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1
Speak to your doctor about your breathing problems. Increased levels of nitric oxide are associated with swelling in the airways, so a pulmonary specialist may test your exhaled nitric oxide levels to check for asthma when other routine tests are not providing answers. Tell your doctor about any breathing difficulties you are having. Ask about the exhaled nitric oxide test, as it may not be available in all doctor's offices or hospitals.^{[5]XTrustworthy SourceAmerican Lung AssociationNonprofit health organization dedicated to improving lung health through education, advocacy, and researchGo to source}
- Your doctor will usually make a separate appointment for your test so you can prepare for it.
- This test may be ordered if you have already been diagnosed with asthma if your doctor is testing your treatment progress or considering a new line of treatment.
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Avoid alcohol and tobacco for 24 hours prior to your test. There are many things that can affect nitric oxide levels in your body, even in small doses. Alcohol and tobacco should be avoided for a full 24 hours before your test. Inform your doctor if you are a regular smoker.^{[6]XResearch source}
- Long-term drinking habits can affect your nitric oxide levels significantly, so tell your doctor if you drink more than 1 drink a day, or drink more than 4-5 drinks a day at least once a month.^{[7]XResearch source}
3
Avoid aerobic exercise for 24 hours before your test. While cardiovascular exercise is excellent for your health, it should be avoided for a full day before your test. Aerobic activity increases the levels of nitric oxide in your body, which may skew the results of your test. Skip any type of workout, including:^{[8]XResearch source}
- Jogging or running
- Bicycling
- Jumping rope
- Rollerblading
- Dancing
- Speed walking
Allergy shots work by introducing allergens into your system to help it build a resistance to them. As a result, an allergy vaccination may affect your nitric oxide levels for 24-48 hours after it is administered. To ensure accurate results, avoid scheduling an allergy shot within 1-2 days of your nitric oxide test.
Consuming food or beverages right before your test can affect the levels of nitric oxide being released. Plan ahead so you can go at least an hour without eating or drinking anything prior to your test appointment. This includes drinking water, which can also skew test results.^{[9]XResearch source}
6
Disclose any medications conditions that may affect your results. Nitric oxide levels in the body may be affected by other health issues and treatments. Disclose any prescription or over-the-counter medications that you are taking. If you are sick with the flu, allergies, or another ailment, tell your doctor to rule out any other causes of your symptoms.^{[10]XResearch source}
- Share the results of any other breathing tests you have undergone in the past.
7
Follow the technician's instructions for breathing during the test. The exhaled nitric oxide test will last about 5 minutes. Allow the technician helping you to put clips on your nose and a mouthpiece in your mouth when you are ready to start the test. Inhale and exhale slowly until the technician or doctor tells you to stop.^{[11]XTrustworthy SourceAmerican Lung AssociationNonprofit health organization dedicated to improving lung health through education, advocacy, and researchGo to source}
- You may have to repeat the test a few times to confirm the results.
- The amount of nitric oxide exhaled into the mouthpiece will be recorded for your test results.

Method 3

Method 3 of 3:

Increasing Your Nitric Oxide Levels

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1
Get at least 30 minutes of cardiovascular exercise every day. Cardiovascular exercise prompts the production of nitric oxide in your body. Aim to do at least 30 minutes of moderate to intense cardio every day. This kind of exercise could include running, biking, swimming, rollerblading, dancing, or sped walking.^{[12]XTrustworthy SourcePubMed CentralJournal archive from the U.S. National Institutes of HealthGo to source}
- If you can't schedule 30 minute sessions of exercise every day, break up the 30 minutes into 10-15 minute blocks.
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Eats foods that are high in nitrogen and protein. Nitrogen produces nitric oxide when metabolized in the body, and can be found in many vegetables. Similarly, most high-protein foods contain arginine, which produces nitric oxide when broken down in the body. Each day, try to add 1-2 servings of foods like:^{[13]XResearch source}
- Kale
- Spinach
- Broccoli
- Brussel sprouts
- Beets
- Legumes
- Nuts
- Beans
- Fish (e.g. salmon)
- Meats (e.g. beef, chicken)
- Cheese
- Eggs
Exposure to UV light prompts the body to unlock its own stores of nitric oxide. Get outside for least 20 minutes a day, during work breaks or for short walks. Wear SPF sunscreen to protect your skin from sun damage.^{[14]XResearch source}